| Research Fields |
Plant Molecular Breeding |
| Research Project |
Genetic improvement of crop production |
| Research Keywords |
rice, brassinosteroid, yielding ability, plant type, quantitative trait locus |
| Research Interests |
My main focus of interest is the genetic improvement of crop production. Several parameters such as plant height, grain number, culm stiffness, tiller number, photosynthesis, root system structure, and stress tolerance are associated with stability and increase in crop yield potential. To achieve genetic improvement of crops, I want to understand the framework of the genetic network regulating these agriculturally important traits. To realize this, we have to combine molecular, genetic, physiological, and biochemical approaches. Through these studies, I want to construct a model for the genetic network controlling agronomical traits and establish an efficient strategy to improve crop productivity. |
| Selected Publications |
Peer-Reviewed Articles 1. Sakamoto T, Y. Morinaka, T. Ohnishi, H. Sunohara, S. Fujioka, M. Ueguchi-Tanaka, M. Mizutani, K. Sakata, S. Takatsuto, S. Yoshida, H. Tanaka, H. Kitano, M. Matsuoka: Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nature Biotechnology 24, 105-109, 2006. 2. Sakamoto T, H. Sakakibara, M. Kojima, Y. Yamamoto, H. Nagasaki, Y. Inukai, Y. Sato, M. Matsuoka M: Ectopic expression of KNOX homeodomain protein induces expression of cytokinin biosynthesis gene in rice. Plant Physiology 142, 54-62, 2006. 3. Sakamoto T, M. Matsuoka: Characterization of CONSTITUTIVE PHOTOMORPHOGENESIS AND DWARFISM homologs in rice (Oryza sativa L.). Journal of Plant Growth Regulation 25, 245-251, 2006. 4. Shimada A, M. Ueguchi-Tanaka, T. Sakamoto, S. Fujioka, S. Takatsuto, S. Yoshida, T. Sazuka, M. Ashikari, M. Matsuoka: The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and is involved in brassinosteroid synthesis. Plnat Journal 48, 390-402, 2006. 5. Morinaka Y, T. Sakamoto, Y. Inukai, M. Agetsuma, H. Kitano, M. Ashikari, M. Matsuoka: Morphological alteration caused by brassinosteroid insensitivity increases the biomass and grain production of rice. Plant Physiology 141, 924-931, 2006. 6. Inukai Y, T. Sakamoto, M. Ueguchi-Tanaka, Y. Shibata, K. Gomi, I. Umemura, Y. Hasegawa, M. Ashikari, H. Kitano, M. Matsuoka: Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling. Plant Cell 17, 1387-1396, 2005. 7. Sakamoto T, K. Miura, H. Itoh, T. Tatsumi, M. Ueguchi-Tanaka, K. Ishiyama, M. Kobayashi, G. K. Agrawal, S. Takeda, K. Abe, A. Miyao, H. Hirochika, H. Kitano, M. Ashikari, M. Matsuoka: An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiology 134, 1642-1653, 2004. 8. Itoh H, T. Tatsumi, T. Sakamoto, K. Otomo, T. Toyomasu, H. Kitano, M. Ashikari, 9. Sakamoto T, Y. Morinaka, K. Ishiyama, M. Kobayashi, H. Itoh, T. Kayano, S. Iwahori, M. Matsuoka, H. Tanaka: Genetic manipulation of gibberellin metabolism in transgenic rice. Nature Biotechnology 21, 909-913, 2003. 10. Kaneko M, H. Itoh, Y. Inukai, T. Sakamoto, M. Ueguchi-Tanaka, M. Ashikari, M. Matsuoka: Where do gibberellins biosynthesis and gibberellin signaling occur in rice plants? Plant Journal 35, 104-115, 2003. 11. Sakai M, T. Sakamoto, T. Saito, M. Matsuoka, H. Tanaka, M. Kobayashi: Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins. Journal of Plant Research 116, 161-164, 2003. 12. Itoh H, M. Ueguchi-Tanaka, T. Sakamoto, T. Kayano, H. Tanaka, M. Ashikari, M. Matsuoka: Modification of rice plant height by suppressing the height-controlling gene, D18, in rice. Breeding Science 52, 215?218, 2002. 13. Sato Y, A. Nishimura, T. Sakamoto, M. Ashikari, M. Matsuoka: Isolation and characterization of a cDNA encoding an orthologue of ROUGH SHEATH2 (OsRS2) from rice. Plant Biotechnology 19, 11-18, 2002. 14. Sakamoto T, N. Kamiya, M. Ueguchi-Tanaka, S. Iwahori, M. Matsuoka: KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem. Genes & Development 15, 581-590, 2001. 15. Sakamoto T, M. Kobayashi, H. Itoh, A. Tagiri, T. Kayano, H. Tanaka, S. Iwahori, M. Matsuoka: Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice. Plant Physiology 125, 1508-1516, 2001. 16. Nagasaki H, T. Sakamoto, Y. Sato, M. Matsuoka: Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15. Plant Cell 13, 2085-2098, 2001. 17. Nishimura A, M. Tamaoki, T. Sakamoto, M. Matsuoka: Over-expression of tobacco knotted1-type class1 homeobox genes alters various leaf morphology. Plant Cell Physiology 41, 583-590, 2000. 18. Sakamoto T, A. Nishimura, M. Tamaoki, M. Kuba, H. Tanaka, S. Iwahori, M. Matsuoka: The conserved KNOX domain mediates specificity of tobacco KNOTTED1-type homeodomain proteins. Plant Cell 11, 1419-1431, 1999. 19. Kusaba S, Y. Kano-Murakami, M. Matsuoka, N. Matsuta, T. Sakamoto, M. Fukumoto: Expression of the rice homeobox gene OSH1 causes morphological changes in transgenic kiwifruit. Journal of Japanese Society for Horticultural Science 68, 482-486, 1999. 20. Sakamoto T, S. Kusaba, Y. Kano-Murakami, M. Fukumoto, S. Iwahori: Two different types of homeobox genes exist in apples. Journal of Japanese Society for Horticultural Science 67, 372-374, 1998. 21. Kusaba S, Y. Kano-Murakami, M. Matsuoka, M. Tamaoki, T. Sakamoto, I. Yamaguchi, M. Fukumoto: Alteration of hormone levels in transgenic tobacco plants overexpressing a rice homeobox gene OSH1. Plant Physiology 116, 471-476, 1998. 22. Kusaba S, M. Fukumoto, C. Honda, I. Yamaguchi, T. Sakamoto, Y. Kano-Murakami Y: Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA 20-oxidase gene expression. Plant Physiology 117, 1179-1184, 1998. Other Publications 1. Sakamoto T.: Phytohormones and rice crop yield: Strategies and opportunities for genetic improvement. Transgenic Research, 15, 399-404, 2006. 2. Sakamoto T, M. Matsuoka: Generating high-yielding varieties by genetic manipulation of plant architecture. Current Opinion in Biotechnology 15, 144-147, 2004. 3. Sakamoto T.: Generating semi-dwarf rice by genetic manipulation of gibberellin metabolism. ISB News Report November 2003, 4-6, 2003. 4. Sakamoto T, Y. Sato, M. Matsuoka: Function of KNOX homeodomain proteins in plant development. Plant Biotechnology 18, 85-92, 2001.
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