Cell Biology, Molecular Genetics, Biochemistry, Biophysics; Elucidation of the molecular mechanism for the biological clock molecular machinery at the atomic level.

• Iwase, R., Imada, K., Hayashi, F., Uzumaki, T., Morishita, M., Onai, K., Furukawa, Y., Namba, K., and Ishiura, M. (2005) Functionally important substructures of circadian clock protein KaiB in unique tetramer complex. J. Bio. Chem., in press.
  

• Onai K, Ishiura M. (2005): PHYTOCLOCK 1 encoding a novel GARP protein esstial for the Arabidopsis circadain clock. Genes Cells. 10:963-972.
  
  
• Okamoto, K., Onai, K., Furusawa, T., and Ishiura, M. (2005) A portable integrated automatic apparatus for the real-time monitoring of bioluminescence in plants. Plant Cell Environ. 28:1305-1315.
  
  
• Kucho, K., Okamoto, K., Tabata, S., Fukuzawa, H., and Ishiura, M. (2005) Identification of novel clock-regulated genes by cDNA macroarray analysis in Chlamydomonas reinhardtii. Plant Mol. Biol. 57:889-906.
  
  
• Okamoto, K. Onai, K., Ezaki, N., Ofuchi, T., and Ishiura, M. (2005) An automated apparatus for the real-time monitoring of bioluminescence in plants. Anal. Biochem. 340:187-192.
  
  
• Okamoto, K., Onai, K., and Ishiura, M. (2005) RAP, an integrated program for monitoring bioluminescence and analyzing circadian rhythms in real time. Anal. Biochem. 340:193-2000.
  
  
• Kucho, K., Aoki, K., Itoh, S., and Ishiura, M. (2005) Improvement of the bioluminescent reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes Genet. Syst. 80:19-23.
  
  
• Kucho, K., Okamoto, K., Tsuchiya, Y., Nomura, S., Nango, M., Kanehisa, M., and Ishiura, M. (2005) Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803. J. Bacteriol. 187: 2190-2199.
  
  
• Kucho, K., Tsuchiya, Y., Okumoto, Y., Harada, M., Yamada, M., and Ishiura, M. (2004) Construction of unmodified oligonucleotide-based microarray in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Genes Genet. Syst. 79: 319-329.
  
  
• Hayashi, F., Ito, N., Uzumaki, T., Iwase, R., Tsuchiya, Y., Yamakawa, H., Morishita, M., Onai, K., Itoh, S., and Ishiura, M. (2004) Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC. J. Biol. Chem. 279: 52331-52337.
  
  
• Kucho, K., Yoneda, H., Harada, M., and Ishiura, M. (2004) Determinants of sensitivity and specificity in spotted DNA microarrays with unmodified oligonucleotides. Genes Genet. Syst. 79: 189-197.
  
  
• Onai, K., Okamoto, K., Nishimoto, H., Morioka, C., Hirano, M., Kami-ike, N., and Ishiura, M. (2004) Large-scale screening of Arabidopsis circadian clock mutants by a high-throughput real-time bioluminescence monitoring system. Plant J. 40: 1-11.
  
  
• Onai, K., Morishita, M., Itoh, S., Okamoto, K., and Ishiura, M. (2004) Circadian rhythms in the thermophilic cyanobacterium Thermosynechococcus elongatus: compensation of period length over a wide temperature range. J. Bacteriol. 186: 4972-4977.
  
  
• Uzumaki, T., Fujita, M., Nakatsu, T., Hayashi, F., Shibata, H., Itoh, N., Kato, H., and Ishiura, M. (2004) Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein. Nature Struct. Mol. Biol. 11: 623-631.
  
  
• Iwase, R., Imada, K., Hayashi, F., Uzumaki, T., Namba, K., and Ishiura, M. (2004) Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Acta Cryst. D 60: 727-729.
  
  
• Hayashi, F., Ito, H., Fujita, M., Iwase, R., Uzumaki, T., and Ishiura, M. (2004) Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC. Biochem. Biophys. Res. Commun. 316: 195-202.
  
  
• Onai, K., Morishita, M., Kaneko, T., Tabata, S., and Ishiura, M. (2004) Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer. Mol. Genet. Genomics 271: 50-59.
  
  
• Hayashi, F., Suzuki, H., Iwase, R., Uzumaki, T., Miyake, A., Shen, J.-R., Imada, K., Furukawa, Y., Yonekura, K., Namba, K., and Ishiura, M. (2003) ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC. Genes Cells 8: 287-296.