Molecular Biology

1) Molecular mechanism of membrane transport protein

2) Structure and function of ion channel and ion transporter

3) Membrane topogenesis of voltage sensor.

4) Plant cell response to enviromental stress

• Matsuda, N., Kobayashi, H., Katoh, H., Ogawa, T., Futatsugi, L., Nakamura, T., Bakker, E.P., and Uozumi, N.　Na⁺-dependent K⁺ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC 6803 and its role in the early phases of cell adaptation to hyperosmotic shock.,J. Biol. Chem., Inpress
  

• Zhang, L., Lee, J-K., John, S. A., Uozumi, N., and Kodama, I., Mechanosensitivity of GIRK channels is mediated by PKC-dependent channel-PIP₂ interaction.,J. Biol. Chem., 279, 7037 - 7047. (2004)
  
  
• Sato, Y., Sakaguchi, M., Goshima, S., Nakamura, T., and Uozumi, N.,Molecular dissection of the contribution of negatively and positively charged residues in S2, S3, and S4 to the final membrane topology of the voltage sensor in the K⁺ channel, KAT1.,J. Biol. Chem., 278, 13227-13234. (2003)
  
  
• Umigai, N., Sato, Y., Mizutani, A., Utsumi, T., Sakaguchi, M., and Uozumi, N. ,Topogenesis of two transmembrane type K⁺ channels, Kir 2.1 and KcsA.,J. Biol. Chem., 278, 40373-40384. (2003)
  
  
• Berthomieu, P., Conejero G., Nublat A., Brackenbury W.L. Lambert C., Savio, C., Uozumi, N., Oiki, S., Yamada, K., Cellier, F., Gosti, F., Simonneau, T., Essah, P. A., Tester, M., Very, A-A, Sentenac, H., and Casse, F.,Functional analysis of AtHKT1 in Arabidopsis shows that Na⁺ recirculation by the phloem is crucial for salt tolerance.,EMBO J., 22, 2004-2014. (2003)
  
  
• Holtmann, G., Bakker, E. P., Uozumi, N., and Bremerl, E.,KtrAB and KtrCD: two K⁺ uptake systems in Bacillus subtilis and their role in the adaptation to hypertonicity.,J Bacteriol., 185, 1289-1298. (2003)
  
  
• Sato, Y., Sakaguchi, M., Goshima, S., Nakamura, T., and Uozumi, N.,Integration of Shaker-type K⁺ channel, KAT1 into the endoplasmic reticulum membrane: Synergistic insertion of voltage sensing segments, S3-S4 and independent insertion of pore-forming segments, S5-P-S6.,Proc. Natl. Acad. Sci. USA., 99, 60-65. (2002)
  
  
• Maser, P., Hosoo, Y., Goshima, S., Horie, T., Eckelman B., Yamada, K.,Yoshida, K., Bakker, E.P., Shinmyo, A., Oiki, S., Schroeder, J. I., and Uozumi, N.,Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants.,Proc. Natl. Acad. Sci. USA., 99, 6428-6433. (2002)
  
  
• Kato, Y., Sakaguchi, M., Mori, Y., Saito, K., Nakamura, T., Bakker, E. P., Sato, Y., Goshima, S., and Uozumi, N.,Evidence in support of a four transmembrane-pore-transmembrane topology model for the Arabidopsis thaliana Na⁺/K⁺ translocating AtHKT1 protein, a member of the superfamily of K⁺ transporters.,Proc. Natl. Acad. Sci. USA., 98, 6488-6493. (2001)
  
  
• Nakamura, T., Fujisaki, Y., Enomoto, H., Nakayama, Y., Takabe, T., Yamaguchi, N., and Uozumi, N.,Residue aspartate-147 from the third transmembrane region of Na⁺/H⁺ antiporter NhaB of Vibrio aliginolyticus plays a role in its activity.,J. Bacteriol., 183, 5762-5767. (2001)